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Electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots
The influence of pulsed electric field (PEF) (0.5–2 kV/cm) treatment of wheatgrass (Triticum aestivum L.) seeds, with different water contents, on antioxidant metabolism in the resultant seedlings was investigated. Imbibing seeds to a water content of 45% or greater prior to PEF treatment increased...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857078/ https://www.ncbi.nlm.nih.gov/pubmed/27147445 http://dx.doi.org/10.1038/srep25306 |
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author | Leong, Sze Ying Burritt, David John Oey, Indrawati |
author_facet | Leong, Sze Ying Burritt, David John Oey, Indrawati |
author_sort | Leong, Sze Ying |
collection | PubMed |
description | The influence of pulsed electric field (PEF) (0.5–2 kV/cm) treatment of wheatgrass (Triticum aestivum L.) seeds, with different water contents, on antioxidant metabolism in the resultant seedlings was investigated. Imbibing seeds to a water content of 45% or greater prior to PEF treatment increased the glutathione level and activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and ascorbate peroxidase in the resultant seedlings, compared to untreated controls. Pre-culture of human intestinal Caco-2 cells with simulated gastrointestinal digests of electrostimulated seedlings enhanced the ability of Caco-2 cells to cope with H(2)O(2)-induced oxidative damage, determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays. The Caco-2 cell MTT and LDH assays correlated better with the increases in seedling glutathione content and antioxidant enzyme activities compared to the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) total antioxidant capacity assay, an assay commonly used to determine the ability of plant extracts to protect cells from oxidative damage. These results demonstrate for the first time that PEF treatment of imbibed seeds can stimulate changes in metabolism in the resultant seedlings, increasing the bioprotective potential of their shoots/sprouts and hence value as functional foods. |
format | Online Article Text |
id | pubmed-4857078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48570782016-05-18 Electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots Leong, Sze Ying Burritt, David John Oey, Indrawati Sci Rep Article The influence of pulsed electric field (PEF) (0.5–2 kV/cm) treatment of wheatgrass (Triticum aestivum L.) seeds, with different water contents, on antioxidant metabolism in the resultant seedlings was investigated. Imbibing seeds to a water content of 45% or greater prior to PEF treatment increased the glutathione level and activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and ascorbate peroxidase in the resultant seedlings, compared to untreated controls. Pre-culture of human intestinal Caco-2 cells with simulated gastrointestinal digests of electrostimulated seedlings enhanced the ability of Caco-2 cells to cope with H(2)O(2)-induced oxidative damage, determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays. The Caco-2 cell MTT and LDH assays correlated better with the increases in seedling glutathione content and antioxidant enzyme activities compared to the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) total antioxidant capacity assay, an assay commonly used to determine the ability of plant extracts to protect cells from oxidative damage. These results demonstrate for the first time that PEF treatment of imbibed seeds can stimulate changes in metabolism in the resultant seedlings, increasing the bioprotective potential of their shoots/sprouts and hence value as functional foods. Nature Publishing Group 2016-05-05 /pmc/articles/PMC4857078/ /pubmed/27147445 http://dx.doi.org/10.1038/srep25306 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Leong, Sze Ying Burritt, David John Oey, Indrawati Electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots |
title | Electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots |
title_full | Electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots |
title_fullStr | Electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots |
title_full_unstemmed | Electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots |
title_short | Electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots |
title_sort | electropriming of wheatgrass seeds using pulsed electric fields enhances antioxidant metabolism and the bioprotective capacity of wheatgrass shoots |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857078/ https://www.ncbi.nlm.nih.gov/pubmed/27147445 http://dx.doi.org/10.1038/srep25306 |
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