Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

In recent years, the assay for transposase-accessible chromatin using sequencing (ATAC-Seq) has become a fundamental tool of epigenomic research. However, it is difficult to perform this technique on frozen samples because freezing cells before extracting nuclei can impair nuclear integrity and alte...

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Detalles Bibliográficos
Autores principales: Milani, Pamela, Escalante-Chong, Renan, Shelley, Brandon C., Patel-Murray, Natasha L., Xin, Xiaofeng, Adam, Miriam, Mandefro, Berhan, Sareen, Dhruv, Svendsen, Clive N., Fraenkel, Ernest
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857123/
https://www.ncbi.nlm.nih.gov/pubmed/27146274
http://dx.doi.org/10.1038/srep25474

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857123/
https://www.ncbi.nlm.nih.gov/pubmed/27146274
http://dx.doi.org/10.1038/srep25474

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