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A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways
Embryonic stem (ES) cells share markers with undifferentiated primordial germ cells (PGCs). Here, we discovered that a cellular state with some molecular markers of male gonocyte induction, including a G1/S phase arrest and upregulation of specific genes such as Nanos2, Tdrd1, Ddx4, Zbtb16 and Plk1s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860597/ https://www.ncbi.nlm.nih.gov/pubmed/27157261 http://dx.doi.org/10.1038/srep25104 |
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author | Moshfegh, Cameron Aires, Lina Kisielow, Malgorzata Vogel, Viola |
author_facet | Moshfegh, Cameron Aires, Lina Kisielow, Malgorzata Vogel, Viola |
author_sort | Moshfegh, Cameron |
collection | PubMed |
description | Embryonic stem (ES) cells share markers with undifferentiated primordial germ cells (PGCs). Here, we discovered that a cellular state with some molecular markers of male gonocyte induction, including a G1/S phase arrest and upregulation of specific genes such as Nanos2, Tdrd1, Ddx4, Zbtb16 and Plk1s1, can be chemically induced in male mouse ES cells in vitro, which we termed gonogenic stimulated transition (GoST). After longer culture of the resulting GoST cells without chemical stimulation, several molecular markers typical for early gonocytes were detected including the early gonocyte marker Tex101. Motivated by previous studies that found multipotency in cell lines derived from neonatal male germ cells in vitro, we then compared the differentiation potential of GoST cells to that of ES cells in vitro. Interestingly, GoST cells showed equal neurogenic, but enhanced cardiogenic and hepatogenic differentiation compared to ES cells in vitro. This work shows for the first time that some important molecular markers of the first developmental sexual differentiation program can be induced in male mouse ES cells in vitro and defines a novel concept to generate cells with enhanced multipotency. |
format | Online Article Text |
id | pubmed-4860597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48605972016-05-20 A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways Moshfegh, Cameron Aires, Lina Kisielow, Malgorzata Vogel, Viola Sci Rep Article Embryonic stem (ES) cells share markers with undifferentiated primordial germ cells (PGCs). Here, we discovered that a cellular state with some molecular markers of male gonocyte induction, including a G1/S phase arrest and upregulation of specific genes such as Nanos2, Tdrd1, Ddx4, Zbtb16 and Plk1s1, can be chemically induced in male mouse ES cells in vitro, which we termed gonogenic stimulated transition (GoST). After longer culture of the resulting GoST cells without chemical stimulation, several molecular markers typical for early gonocytes were detected including the early gonocyte marker Tex101. Motivated by previous studies that found multipotency in cell lines derived from neonatal male germ cells in vitro, we then compared the differentiation potential of GoST cells to that of ES cells in vitro. Interestingly, GoST cells showed equal neurogenic, but enhanced cardiogenic and hepatogenic differentiation compared to ES cells in vitro. This work shows for the first time that some important molecular markers of the first developmental sexual differentiation program can be induced in male mouse ES cells in vitro and defines a novel concept to generate cells with enhanced multipotency. Nature Publishing Group 2016-05-09 /pmc/articles/PMC4860597/ /pubmed/27157261 http://dx.doi.org/10.1038/srep25104 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Moshfegh, Cameron Aires, Lina Kisielow, Malgorzata Vogel, Viola A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways |
title | A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways |
title_full | A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways |
title_fullStr | A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways |
title_full_unstemmed | A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways |
title_short | A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways |
title_sort | gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860597/ https://www.ncbi.nlm.nih.gov/pubmed/27157261 http://dx.doi.org/10.1038/srep25104 |
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