Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in Clostridium pasteurianum for markerless genome editing. Since 74% of species harbor CRISPR-Cas loci in Clostridium, we also...

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Detalles Bibliográficos
Autores principales: Pyne, Michael E., Bruder, Mark R., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860712/
https://www.ncbi.nlm.nih.gov/pubmed/27157668
http://dx.doi.org/10.1038/srep25666

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860712/
https://www.ncbi.nlm.nih.gov/pubmed/27157668
http://dx.doi.org/10.1038/srep25666

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