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Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in Clostridium pasteurianum for markerless genome editing. Since 74% of species harbor CRISPR-Cas loci in Clostridium, we also...

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Detalles Bibliográficos
Autores principales:	Pyne, Michael E., Bruder, Mark R., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860712/ https://www.ncbi.nlm.nih.gov/pubmed/27157668 http://dx.doi.org/10.1038/srep25666

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