A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons

Retroviruses expressing a fluorescent protein, Cas9, and a small guide RNA are used to mimic nonsense PTEN mutations from autism patients in developing mouse neurons. We compare the cellular phenotype elicited by CRISPR-Cas9 to those elicited using shRNA or Cre/Lox technologies and find that knockdo...

Descripción completa

Detalles Bibliográficos
Autores principales: Williams, Michael R., Fricano-Kugler, Catherine J., Getz, Stephanie A., Skelton, Patrick D., Lee, Jeonghoon, Rizzuto, Christian P., Geller, Joseph S., Li, Meijie, Luikart, Bryan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861960/
https://www.ncbi.nlm.nih.gov/pubmed/27161796
http://dx.doi.org/10.1038/srep25611

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861960/
https://www.ncbi.nlm.nih.gov/pubmed/27161796
http://dx.doi.org/10.1038/srep25611

Ejemplares similares