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A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons

Retroviruses expressing a fluorescent protein, Cas9, and a small guide RNA are used to mimic nonsense PTEN mutations from autism patients in developing mouse neurons. We compare the cellular phenotype elicited by CRISPR-Cas9 to those elicited using shRNA or Cre/Lox technologies and find that knockdo...

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Detalles Bibliográficos
Autores principales:	Williams, Michael R., Fricano-Kugler, Catherine J., Getz, Stephanie A., Skelton, Patrick D., Lee, Jeonghoon, Rizzuto, Christian P., Geller, Joseph S., Li, Meijie, Luikart, Bryan W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861960/ https://www.ncbi.nlm.nih.gov/pubmed/27161796 http://dx.doi.org/10.1038/srep25611

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