2C-Cas9: a versatile tool for clonal analysis of gene function

CRISPR/Cas9-mediated targeted mutagenesis allows efficient generation of loss-of-function alleles in zebrafish. To date, this technology has been primarily used to generate genetic knockout animals. Nevertheless, the study of the function of certain loci might require tight spatiotemporal control of...

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Detalles Bibliográficos
Autores principales: Di Donato, Vincenzo, De Santis, Flavia, Auer, Thomas O., Testa, Noé, Sánchez-Iranzo, Héctor, Mercader, Nadia, Concordet, Jean-Paul, Del Bene, Filippo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864464/
https://www.ncbi.nlm.nih.gov/pubmed/26957310
http://dx.doi.org/10.1101/gr.196170.115

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864464/
https://www.ncbi.nlm.nih.gov/pubmed/26957310
http://dx.doi.org/10.1101/gr.196170.115

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