Optimized Illumina PCR-free library preparation for bacterial whole genome sequencing and analysis of factors influencing de novo assembly

BACKGROUND: Next-generation sequencing (NGS) technology has paved the way for rapid and cost-efficient de novo sequencing of bacterial genomes. In particular, the introduction of PCR-free library preparation procedures (LPPs) lead to major improvements as PCR bias is largely reduced. However, in ord...

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Detalles Bibliográficos
Autores principales: Huptas, Christopher, Scherer, Siegfried, Wenning, Mareike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864918/
https://www.ncbi.nlm.nih.gov/pubmed/27176120
http://dx.doi.org/10.1186/s13104-016-2072-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864918/
https://www.ncbi.nlm.nih.gov/pubmed/27176120
http://dx.doi.org/10.1186/s13104-016-2072-9

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