Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader

Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ß-d-galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by...

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Detalles Bibliográficos
Autores principales: Schaefer, Jorrit, Jovanovic, Goran, Kotta-Loizou, Ioly, Buck, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865525/
https://www.ncbi.nlm.nih.gov/pubmed/27036618
http://dx.doi.org/10.1016/j.ab.2016.03.017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865525/
https://www.ncbi.nlm.nih.gov/pubmed/27036618
http://dx.doi.org/10.1016/j.ab.2016.03.017

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