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Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria

Dissecting the complexities of branched peptide-lipopolysaccharides (LPS) interactions provide rationale for the development of non-cytotoxic antibiotic adjuvants. Using various biophysical methods, we show that the branched peptide, B2088, binds to lipid A and disrupts the supramolecular organizati...

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Autores principales: Lakshminarayanan, Rajamani, Tan, Wei Xiang, Aung, Thet Tun, Goh, Eunice Tze Leng, Muruganantham, Nandhakumar, Li, Jianguo, Chang, Jamie Ya Ting, Dikshit, Neha, Saraswathi, Padmanabhan, Lim, Rayne Rui, Kang, Tse Siang, Balamuralidhar, Vanniarajan, Sukumaran, Bindu, Verma, Chandra S., Sivaraman, Jayaraman, Chaurasia, Shyam Sunder, Liu, Shouping, Beuerman, Roger W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865820/
https://www.ncbi.nlm.nih.gov/pubmed/27174567
http://dx.doi.org/10.1038/srep25905
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author Lakshminarayanan, Rajamani
Tan, Wei Xiang
Aung, Thet Tun
Goh, Eunice Tze Leng
Muruganantham, Nandhakumar
Li, Jianguo
Chang, Jamie Ya Ting
Dikshit, Neha
Saraswathi, Padmanabhan
Lim, Rayne Rui
Kang, Tse Siang
Balamuralidhar, Vanniarajan
Sukumaran, Bindu
Verma, Chandra S.
Sivaraman, Jayaraman
Chaurasia, Shyam Sunder
Liu, Shouping
Beuerman, Roger W.
author_facet Lakshminarayanan, Rajamani
Tan, Wei Xiang
Aung, Thet Tun
Goh, Eunice Tze Leng
Muruganantham, Nandhakumar
Li, Jianguo
Chang, Jamie Ya Ting
Dikshit, Neha
Saraswathi, Padmanabhan
Lim, Rayne Rui
Kang, Tse Siang
Balamuralidhar, Vanniarajan
Sukumaran, Bindu
Verma, Chandra S.
Sivaraman, Jayaraman
Chaurasia, Shyam Sunder
Liu, Shouping
Beuerman, Roger W.
author_sort Lakshminarayanan, Rajamani
collection PubMed
description Dissecting the complexities of branched peptide-lipopolysaccharides (LPS) interactions provide rationale for the development of non-cytotoxic antibiotic adjuvants. Using various biophysical methods, we show that the branched peptide, B2088, binds to lipid A and disrupts the supramolecular organization of LPS. The disruption of outer membrane in an intact bacterium was demonstrated by fluorescence spectroscopy and checkerboard assays, the latter confirming strong to moderate synergism between B2088 and various classes of antibiotics. The potency of synergistic combinations of B2088 and antibiotics was further established by time-kill kinetics, mammalian cell culture infections model and in vivo model of bacterial keratitis. Importantly, B2088 did not show any cytotoxicity to corneal epithelial cells for at least 96 h continuous exposure or hemolytic activity even at 20 mg/ml. Peptide congeners containing norvaline, phenylalanine and tyrosine (instead of valine in B2088) displayed better synergism compared to other substitutions. We propose that high affinity and subsequent disruption of the supramolecular assembly of LPS by the branched peptides are vital for the development of non-cytotoxic antibiotic adjuvants that can enhance the accessibility of conventional antibiotics to the intracellular targets, decrease the antibiotic consumption and holds promise in averting antibiotic resistance.
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spelling pubmed-48658202016-05-23 Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria Lakshminarayanan, Rajamani Tan, Wei Xiang Aung, Thet Tun Goh, Eunice Tze Leng Muruganantham, Nandhakumar Li, Jianguo Chang, Jamie Ya Ting Dikshit, Neha Saraswathi, Padmanabhan Lim, Rayne Rui Kang, Tse Siang Balamuralidhar, Vanniarajan Sukumaran, Bindu Verma, Chandra S. Sivaraman, Jayaraman Chaurasia, Shyam Sunder Liu, Shouping Beuerman, Roger W. Sci Rep Article Dissecting the complexities of branched peptide-lipopolysaccharides (LPS) interactions provide rationale for the development of non-cytotoxic antibiotic adjuvants. Using various biophysical methods, we show that the branched peptide, B2088, binds to lipid A and disrupts the supramolecular organization of LPS. The disruption of outer membrane in an intact bacterium was demonstrated by fluorescence spectroscopy and checkerboard assays, the latter confirming strong to moderate synergism between B2088 and various classes of antibiotics. The potency of synergistic combinations of B2088 and antibiotics was further established by time-kill kinetics, mammalian cell culture infections model and in vivo model of bacterial keratitis. Importantly, B2088 did not show any cytotoxicity to corneal epithelial cells for at least 96 h continuous exposure or hemolytic activity even at 20 mg/ml. Peptide congeners containing norvaline, phenylalanine and tyrosine (instead of valine in B2088) displayed better synergism compared to other substitutions. We propose that high affinity and subsequent disruption of the supramolecular assembly of LPS by the branched peptides are vital for the development of non-cytotoxic antibiotic adjuvants that can enhance the accessibility of conventional antibiotics to the intracellular targets, decrease the antibiotic consumption and holds promise in averting antibiotic resistance. Nature Publishing Group 2016-05-13 /pmc/articles/PMC4865820/ /pubmed/27174567 http://dx.doi.org/10.1038/srep25905 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lakshminarayanan, Rajamani
Tan, Wei Xiang
Aung, Thet Tun
Goh, Eunice Tze Leng
Muruganantham, Nandhakumar
Li, Jianguo
Chang, Jamie Ya Ting
Dikshit, Neha
Saraswathi, Padmanabhan
Lim, Rayne Rui
Kang, Tse Siang
Balamuralidhar, Vanniarajan
Sukumaran, Bindu
Verma, Chandra S.
Sivaraman, Jayaraman
Chaurasia, Shyam Sunder
Liu, Shouping
Beuerman, Roger W.
Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria
title Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria
title_full Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria
title_fullStr Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria
title_full_unstemmed Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria
title_short Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria
title_sort branched peptide, b2088, disrupts the supramolecular organization of lipopolysaccharides and sensitizes the gram-negative bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865820/
https://www.ncbi.nlm.nih.gov/pubmed/27174567
http://dx.doi.org/10.1038/srep25905
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