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Intensity Weighted Subtraction Microscopy Approach for Image Contrast and Resolution Enhancement

We propose and demonstrate a novel subtraction microscopy algorithm, exploiting fluorescence emission difference or switching laser mode and their derivatives for image enhancement. The key novelty of the proposed approach lies in the weighted subtraction coefficient, adjusted pixel-by-pixel with re...

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Detalles Bibliográficos
Autores principales: Korobchevskaya, Kseniya, Peres, Chiara, Li, Zhibin, Antipov, Alexei, Sheppard, Colin J. R., Diaspro, Alberto, Bianchini, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865876/
https://www.ncbi.nlm.nih.gov/pubmed/27174367
http://dx.doi.org/10.1038/srep25816