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Degradation Parameters from Pulse-Chase Experiments

Pulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of th...

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Detalles Bibliográficos
Autores principales: Sin, Celine, Chiarugi, Davide, Valleriani, Angelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868333/
https://www.ncbi.nlm.nih.gov/pubmed/27182698
http://dx.doi.org/10.1371/journal.pone.0155028