Glycans affect DNA extraction and induce substantial differences in gut metagenomic studies

Exopolysaccharides produced by bacterial species and present in feces are extremely inhibitory to DNA restriction and can cause discrepancies in metagenomic studies. We determined the effects of different DNA extraction methods on the apparent composition of the gut microbiota using Illumina MiSeq d...

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Detalles Bibliográficos
Autores principales: Angelakis, Emmanouil, Bachar, Dipankar, Henrissat, Bernard, Armougom, Fabrice, Audoly, Gilles, Lagier, Jean-Christophe, Robert, Catherine, Raoult, Didier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870698/
https://www.ncbi.nlm.nih.gov/pubmed/27188959
http://dx.doi.org/10.1038/srep26276

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870698/
https://www.ncbi.nlm.nih.gov/pubmed/27188959
http://dx.doi.org/10.1038/srep26276

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