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Characterisation of the effects of optical aberrations in single molecule techniques

Optical aberrations degrade image quality in fluorescence microscopy, including for single-molecule based techniques. These depend on post-processing to localize individual molecules in an image series. Using simulated data, we show the impact of optical aberrations on localization success, accuracy...

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Detalles Bibliográficos
Autores principales: Coles, Benjamin C., Webb, Stephen E. D., Schwartz, Noah, Rolfe, Daniel J., Martin-Fernandez, Marisa, Lo Schiavo, Valentina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871079/
https://www.ncbi.nlm.nih.gov/pubmed/27231619
http://dx.doi.org/10.1364/BOE.7.001755