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Characterisation of the effects of optical aberrations in single molecule techniques

Optical aberrations degrade image quality in fluorescence microscopy, including for single-molecule based techniques. These depend on post-processing to localize individual molecules in an image series. Using simulated data, we show the impact of optical aberrations on localization success, accuracy...

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Detalles Bibliográficos
Autores principales:	Coles, Benjamin C., Webb, Stephen E. D., Schwartz, Noah, Rolfe, Daniel J., Martin-Fernandez, Marisa, Lo Schiavo, Valentina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Optical Society of America 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871079/ https://www.ncbi.nlm.nih.gov/pubmed/27231619 http://dx.doi.org/10.1364/BOE.7.001755

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