Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene

Green fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence. This coverage dip was present w...

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Detalles Bibliográficos
Autores principales: Van den Hoecke, Silvie, Verhelst, Judith, Saelens, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872057/
https://www.ncbi.nlm.nih.gov/pubmed/27193250
http://dx.doi.org/10.1038/srep26314

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872057/
https://www.ncbi.nlm.nih.gov/pubmed/27193250
http://dx.doi.org/10.1038/srep26314

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