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Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene
Green fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence. This coverage dip was present w...
Autores principales: | Van den Hoecke, Silvie, Verhelst, Judith, Saelens, Xavier |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872057/ https://www.ncbi.nlm.nih.gov/pubmed/27193250 http://dx.doi.org/10.1038/srep26314 |
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