Overcoming the Refractory Expression of Secreted Recombinant Proteins in Mammalian Cells through Modification of the Signal Peptide and Adjacent Amino Acids

The expression and subsequent purification of mammalian recombinant proteins is of critical importance to many areas of biological science. To maintain the appropriate tertiary structure and post-translational modifications of such proteins, transient mammalian expression systems are often adopted....

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Detalles Bibliográficos
Autores principales: Güler-Gane, Gülin, Kidd, Sara, Sridharan, Sudharsan, Vaughan, Tristan J., Wilkinson, Trevor C. I., Tigue, Natalie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873207/
https://www.ncbi.nlm.nih.gov/pubmed/27195765
http://dx.doi.org/10.1371/journal.pone.0155340

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873207/
https://www.ncbi.nlm.nih.gov/pubmed/27195765
http://dx.doi.org/10.1371/journal.pone.0155340

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