Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase

We developed a novel approach to assess tumor vascularity using recombinant Gaussia luciferase (rGluc) protein and bioluminescence imaging. Upon intravenous injection of rGluc followed by its substrate coelenterazine, non-invasive visualization of tumor vascularity by bioluminescence imaging was pos...

Descripción completa

Detalles Bibliográficos
Autores principales: Kantar, Rami S., Lashgari, Ghazal, Tabet, Elie I., Lewandrowski, Grant K., Carvalho, Litia A., Tannous, Bakhos A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873808/
https://www.ncbi.nlm.nih.gov/pubmed/27198044
http://dx.doi.org/10.1038/srep26353
_version_ 1782432945402806272
author Kantar, Rami S.
Lashgari, Ghazal
Tabet, Elie I.
Lewandrowski, Grant K.
Carvalho, Litia A.
Tannous, Bakhos A.
author_facet Kantar, Rami S.
Lashgari, Ghazal
Tabet, Elie I.
Lewandrowski, Grant K.
Carvalho, Litia A.
Tannous, Bakhos A.
author_sort Kantar, Rami S.
collection PubMed
description We developed a novel approach to assess tumor vascularity using recombinant Gaussia luciferase (rGluc) protein and bioluminescence imaging. Upon intravenous injection of rGluc followed by its substrate coelenterazine, non-invasive visualization of tumor vascularity by bioluminescence imaging was possible. We applied this method for longitudinal monitoring of tumor vascularity in response to the anti-angiogenic drug tivozanib. This simple and sensitive method could be extended to image blood vessels/vasculature in many different fields.
format Online
Article
Text
id pubmed-4873808
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-48738082016-06-02 Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase Kantar, Rami S. Lashgari, Ghazal Tabet, Elie I. Lewandrowski, Grant K. Carvalho, Litia A. Tannous, Bakhos A. Sci Rep Article We developed a novel approach to assess tumor vascularity using recombinant Gaussia luciferase (rGluc) protein and bioluminescence imaging. Upon intravenous injection of rGluc followed by its substrate coelenterazine, non-invasive visualization of tumor vascularity by bioluminescence imaging was possible. We applied this method for longitudinal monitoring of tumor vascularity in response to the anti-angiogenic drug tivozanib. This simple and sensitive method could be extended to image blood vessels/vasculature in many different fields. Nature Publishing Group 2016-05-20 /pmc/articles/PMC4873808/ /pubmed/27198044 http://dx.doi.org/10.1038/srep26353 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kantar, Rami S.
Lashgari, Ghazal
Tabet, Elie I.
Lewandrowski, Grant K.
Carvalho, Litia A.
Tannous, Bakhos A.
Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase
title Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase
title_full Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase
title_fullStr Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase
title_full_unstemmed Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase
title_short Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase
title_sort imaging tumor vascularity and response to anti-angiogenic therapy using gaussia luciferase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873808/
https://www.ncbi.nlm.nih.gov/pubmed/27198044
http://dx.doi.org/10.1038/srep26353
work_keys_str_mv AT kantarramis imagingtumorvascularityandresponsetoantiangiogenictherapyusinggaussialuciferase
AT lashgarighazal imagingtumorvascularityandresponsetoantiangiogenictherapyusinggaussialuciferase
AT tabeteliei imagingtumorvascularityandresponsetoantiangiogenictherapyusinggaussialuciferase
AT lewandrowskigrantk imagingtumorvascularityandresponsetoantiangiogenictherapyusinggaussialuciferase
AT carvalholitiaa imagingtumorvascularityandresponsetoantiangiogenictherapyusinggaussialuciferase
AT tannousbakhosa imagingtumorvascularityandresponsetoantiangiogenictherapyusinggaussialuciferase

Ejemplares similares