Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells

Many key regulatory proteins in bacteria are present in too low numbers to be detected with conventional methods, which poses a particular challenge for single-cell analyses because such proteins can contribute greatly to phenotypic heterogeneity. Here we develop a microfluidics-based platform that...

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Detalles Bibliográficos
Autores principales: Okumus, Burak, Landgraf, Dirk, Lai, Ghee Chuan, Bakhsi, Somenath, Arias-Castro, Juan Carlos, Yildiz, Sadik, Huh, Dann, Fernandez-Lopez, Raul, Peterson, Celeste N., Toprak, Erdal, El Karoui, Meriem, Paulsson, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873973/
https://www.ncbi.nlm.nih.gov/pubmed/27189321
http://dx.doi.org/10.1038/ncomms11641

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873973/
https://www.ncbi.nlm.nih.gov/pubmed/27189321
http://dx.doi.org/10.1038/ncomms11641

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