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Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia

BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug treatments. The complexity of human CNS means it un...

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Detalles Bibliográficos
Autores principales: Dean, Brian, Udawela, Madhara, Scarr, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875643/
https://www.ncbi.nlm.nih.gov/pubmed/27206773
http://dx.doi.org/10.1186/s12888-016-0855-0