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Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia
BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug treatments. The complexity of human CNS means it un...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875643/ https://www.ncbi.nlm.nih.gov/pubmed/27206773 http://dx.doi.org/10.1186/s12888-016-0855-0 |