High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG

Adenylate kinase (AK) from Escherichia coli was used as both solubility and affinity tag for recombinant protein production. When fused to the N-terminus of a target protein, an AK fusion protein could be expressed in soluble form and purified to near homogeneity in a single step from Blue-Sepherose...

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Detalles Bibliográficos
Autores principales: Luo, Dan, Wen, Caixia, Zhao, Rongchuan, Liu, Xinyu, Liu, Xinxin, Cui, Jingjing, Liang, Joshua G., Liang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877045/
https://www.ncbi.nlm.nih.gov/pubmed/27214237
http://dx.doi.org/10.1371/journal.pone.0156106

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877045/
https://www.ncbi.nlm.nih.gov/pubmed/27214237
http://dx.doi.org/10.1371/journal.pone.0156106

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