RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses

A fluent method for gene targeting to establish protein tagged and ligand inducible conditional loss-of-function alleles is described. We couple new recombineering applications for one-step cloning of gRNA oligonucleotides and rapid generation of short-arm (~1 kb) targeting constructs with the power...

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Detalles Bibliográficos
Autores principales: Baker, Oliver, Gupta, Ashish, Obst, Mandy, Zhang, Youming, Anastassiadis, Konstantinos, Fu, Jun, Stewart, A. Francis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877586/
https://www.ncbi.nlm.nih.gov/pubmed/27216209
http://dx.doi.org/10.1038/srep25529

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877586/
https://www.ncbi.nlm.nih.gov/pubmed/27216209
http://dx.doi.org/10.1038/srep25529

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