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Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation

Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, inclu...

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Autores principales: Xiong, Yujing, Wang, Enyin, Huang, Yan, Guo, Xiaoyi, Yu, Yiping, Du, Qingyun, Ding, Xiaoyan, Sun, Yingpu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879152/
https://www.ncbi.nlm.nih.gov/pubmed/27059868
http://dx.doi.org/10.1007/s12015-016-9650-z
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author Xiong, Yujing
Wang, Enyin
Huang, Yan
Guo, Xiaoyi
Yu, Yiping
Du, Qingyun
Ding, Xiaoyan
Sun, Yingpu
author_facet Xiong, Yujing
Wang, Enyin
Huang, Yan
Guo, Xiaoyi
Yu, Yiping
Du, Qingyun
Ding, Xiaoyan
Sun, Yingpu
author_sort Xiong, Yujing
collection PubMed
description Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, including DNA methylation and histone modification, plays an essential role in the development and differentiation of hESCs. Lysine-specific demethylase 1 (LSD1), a well-characterized histone-modifying enzyme, demethylates dimethylated histone H3 lysine 4 (H3K4) through a flavin adenine dinucleotide (FAD)-dependent oxidative reaction. LSD1 affects the growth and differentiation of human and mouse ES cells, and the deletion of this gene in mice leads to embryonic lethality. Here, we investigated the functional role of LSD1 during the adipogenic differentiation of hESCs involving the demethylation of H3K4. We also found that treating hESCs with the LSD1 inhibitor CBB1007 promotes the adipogenic differentiation of hESCs.
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spelling pubmed-48791522016-06-21 Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation Xiong, Yujing Wang, Enyin Huang, Yan Guo, Xiaoyi Yu, Yiping Du, Qingyun Ding, Xiaoyan Sun, Yingpu Stem Cell Rev Article Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, including DNA methylation and histone modification, plays an essential role in the development and differentiation of hESCs. Lysine-specific demethylase 1 (LSD1), a well-characterized histone-modifying enzyme, demethylates dimethylated histone H3 lysine 4 (H3K4) through a flavin adenine dinucleotide (FAD)-dependent oxidative reaction. LSD1 affects the growth and differentiation of human and mouse ES cells, and the deletion of this gene in mice leads to embryonic lethality. Here, we investigated the functional role of LSD1 during the adipogenic differentiation of hESCs involving the demethylation of H3K4. We also found that treating hESCs with the LSD1 inhibitor CBB1007 promotes the adipogenic differentiation of hESCs. Springer US 2016-04-08 2016 /pmc/articles/PMC4879152/ /pubmed/27059868 http://dx.doi.org/10.1007/s12015-016-9650-z Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Xiong, Yujing
Wang, Enyin
Huang, Yan
Guo, Xiaoyi
Yu, Yiping
Du, Qingyun
Ding, Xiaoyan
Sun, Yingpu
Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation
title Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation
title_full Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation
title_fullStr Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation
title_full_unstemmed Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation
title_short Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation
title_sort inhibition of lysine-specific demethylase-1 (lsd1/kdm1a) promotes the adipogenic differentiation of hescs through h3k4 methylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879152/
https://www.ncbi.nlm.nih.gov/pubmed/27059868
http://dx.doi.org/10.1007/s12015-016-9650-z
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