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Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses

Superresolution fluorescence microscopy of multiple fluorophores still requires development. Here we present simultaneous three-colour stimulated emission depletion (STED) nanoscopy relying on a single STED beam at 620 nm. Toggling the STED beam between two or more power levels (“multilevelSTED”) op...

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Detalles Bibliográficos
Autores principales: Sidenstein, Sven C., D’Este, Elisa, Böhm, Marvin J., Danzl, Johann G., Belov, Vladimir N., Hell, Stefan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879624/
https://www.ncbi.nlm.nih.gov/pubmed/27220554
http://dx.doi.org/10.1038/srep26725