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Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses
Superresolution fluorescence microscopy of multiple fluorophores still requires development. Here we present simultaneous three-colour stimulated emission depletion (STED) nanoscopy relying on a single STED beam at 620 nm. Toggling the STED beam between two or more power levels (“multilevelSTED”) op...
Autores principales: | Sidenstein, Sven C., D’Este, Elisa, Böhm, Marvin J., Danzl, Johann G., Belov, Vladimir N., Hell, Stefan W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879624/ https://www.ncbi.nlm.nih.gov/pubmed/27220554 http://dx.doi.org/10.1038/srep26725 |
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