Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection

Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have rectified this anomaly by genome engineering. The va...

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Autores principales: Kelly, Michelle L., Ng, Yen Kuan, Cartman, Stephen T., Collery, Mark M., Cockayne, Alan, Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879870/
https://www.ncbi.nlm.nih.gov/pubmed/26946361
http://dx.doi.org/10.1016/j.anaerobe.2016.02.011
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author Kelly, Michelle L.
Ng, Yen Kuan
Cartman, Stephen T.
Collery, Mark M.
Cockayne, Alan
Minton, Nigel P.
author_facet Kelly, Michelle L.
Ng, Yen Kuan
Cartman, Stephen T.
Collery, Mark M.
Cockayne, Alan
Minton, Nigel P.
author_sort Kelly, Michelle L.
collection PubMed
description Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have rectified this anomaly by genome engineering. The variant created (CRG20291) represents an ideal control strain for virulence assays of ClosTron mutants.
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spelling pubmed-48798702016-06-06 Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection Kelly, Michelle L. Ng, Yen Kuan Cartman, Stephen T. Collery, Mark M. Cockayne, Alan Minton, Nigel P. Anaerobe Article Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have rectified this anomaly by genome engineering. The variant created (CRG20291) represents an ideal control strain for virulence assays of ClosTron mutants. Academic Press 2016-06 /pmc/articles/PMC4879870/ /pubmed/26946361 http://dx.doi.org/10.1016/j.anaerobe.2016.02.011 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kelly, Michelle L.
Ng, Yen Kuan
Cartman, Stephen T.
Collery, Mark M.
Cockayne, Alan
Minton, Nigel P.
Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection
title Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection
title_full Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection
title_fullStr Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection
title_full_unstemmed Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection
title_short Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection
title_sort improving the reproducibility of the nap1/b1/027 epidemic strain r20291 in the hamster model of infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879870/
https://www.ncbi.nlm.nih.gov/pubmed/26946361
http://dx.doi.org/10.1016/j.anaerobe.2016.02.011
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