Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucle...

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Detalles Bibliográficos
Autores principales: Isaac, R Stefan, Jiang, Fuguo, Doudna, Jennifer A, Lim, Wendell A, Narlikar, Geeta J, Almeida, Ricardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880442/
https://www.ncbi.nlm.nih.gov/pubmed/27130520
http://dx.doi.org/10.7554/eLife.13450

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880442/
https://www.ncbi.nlm.nih.gov/pubmed/27130520
http://dx.doi.org/10.7554/eLife.13450

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