Downregulation of Runx2 by 1,25-Dihydroxyvitamin D(3) Induces the Transdifferentiation of Osteoblasts to Adipocytes

1,25-Dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) indirectly stimulates bone formation, but little is known about its direct effect on bone formation. In this study, we observed that 1,25(OH)(2)D(3) enhances adipocyte differentiation, but inhibits osteoblast differentiation during osteogenesis. The posit...

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Autores principales: Kim, Jung Ha, Seong, Semun, Kim, Kabsun, Kim, Inyoung, Jeong, Byung-Chul, Kim, Nacksung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881589/
https://www.ncbi.nlm.nih.gov/pubmed/27213351
http://dx.doi.org/10.3390/ijms17050770
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author Kim, Jung Ha
Seong, Semun
Kim, Kabsun
Kim, Inyoung
Jeong, Byung-Chul
Kim, Nacksung
author_facet Kim, Jung Ha
Seong, Semun
Kim, Kabsun
Kim, Inyoung
Jeong, Byung-Chul
Kim, Nacksung
author_sort Kim, Jung Ha
collection PubMed
description 1,25-Dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) indirectly stimulates bone formation, but little is known about its direct effect on bone formation. In this study, we observed that 1,25(OH)(2)D(3) enhances adipocyte differentiation, but inhibits osteoblast differentiation during osteogenesis. The positive role of 1,25(OH)(2)D(3) in adipocyte differentiation was confirmed when murine osteoblasts were cultured in adipogenic medium. Additionally, 1,25(OH)(2)D(3) enhanced the expression of adipocyte marker genes, but inhibited the expression of osteoblast marker genes in osteoblasts. The inhibition of osteoblast differentiation and promotion of adipocyte differentiation mediated by 1,25(OH)(2)D(3) were compensated by Runx2 overexpression. Our results suggest that 1,25(OH)(2)D(3) induces the transdifferentiation of osteoblasts to adipocytes via Runx2 downregulation in osteoblasts.
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spelling pubmed-48815892016-05-27 Downregulation of Runx2 by 1,25-Dihydroxyvitamin D(3) Induces the Transdifferentiation of Osteoblasts to Adipocytes Kim, Jung Ha Seong, Semun Kim, Kabsun Kim, Inyoung Jeong, Byung-Chul Kim, Nacksung Int J Mol Sci Article 1,25-Dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) indirectly stimulates bone formation, but little is known about its direct effect on bone formation. In this study, we observed that 1,25(OH)(2)D(3) enhances adipocyte differentiation, but inhibits osteoblast differentiation during osteogenesis. The positive role of 1,25(OH)(2)D(3) in adipocyte differentiation was confirmed when murine osteoblasts were cultured in adipogenic medium. Additionally, 1,25(OH)(2)D(3) enhanced the expression of adipocyte marker genes, but inhibited the expression of osteoblast marker genes in osteoblasts. The inhibition of osteoblast differentiation and promotion of adipocyte differentiation mediated by 1,25(OH)(2)D(3) were compensated by Runx2 overexpression. Our results suggest that 1,25(OH)(2)D(3) induces the transdifferentiation of osteoblasts to adipocytes via Runx2 downregulation in osteoblasts. MDPI 2016-05-19 /pmc/articles/PMC4881589/ /pubmed/27213351 http://dx.doi.org/10.3390/ijms17050770 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Jung Ha
Seong, Semun
Kim, Kabsun
Kim, Inyoung
Jeong, Byung-Chul
Kim, Nacksung
Downregulation of Runx2 by 1,25-Dihydroxyvitamin D(3) Induces the Transdifferentiation of Osteoblasts to Adipocytes
title Downregulation of Runx2 by 1,25-Dihydroxyvitamin D(3) Induces the Transdifferentiation of Osteoblasts to Adipocytes
title_full Downregulation of Runx2 by 1,25-Dihydroxyvitamin D(3) Induces the Transdifferentiation of Osteoblasts to Adipocytes
title_fullStr Downregulation of Runx2 by 1,25-Dihydroxyvitamin D(3) Induces the Transdifferentiation of Osteoblasts to Adipocytes
title_full_unstemmed Downregulation of Runx2 by 1,25-Dihydroxyvitamin D(3) Induces the Transdifferentiation of Osteoblasts to Adipocytes
title_short Downregulation of Runx2 by 1,25-Dihydroxyvitamin D(3) Induces the Transdifferentiation of Osteoblasts to Adipocytes
title_sort downregulation of runx2 by 1,25-dihydroxyvitamin d(3) induces the transdifferentiation of osteoblasts to adipocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881589/
https://www.ncbi.nlm.nih.gov/pubmed/27213351
http://dx.doi.org/10.3390/ijms17050770
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