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Inherent bacterial DNA contamination of extraction and sequencing reagents may affect interpretation of microbiota in low bacterial biomass samples

BACKGROUND: The advent and use of highly sensitive molecular biology techniques to explore the microbiota and microbiome in environmental and tissue samples have detected the presence of contaminating microbial DNA within reagents. These microbial DNA contaminants may distort taxonomic distributions...

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Detalles Bibliográficos
Autores principales: Glassing, Angela, Dowd, Scot E., Galandiuk, Susan, Davis, Brian, Chiodini, Rodrick J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882852/
https://www.ncbi.nlm.nih.gov/pubmed/27239228
http://dx.doi.org/10.1186/s13099-016-0103-7