Cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells

A variety of nanomaterials have been developed for ocular diseases. The ability of these nanomaterials to pass through the blood-ocular barrier and their biocompatibility are essential characteristics that must be considered. Bacterial magnetosomes (BMs) are a type of biogenic magnetic nanomaterials...

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Autores principales: Qi, Lei, Lv, Xiujuan, Zhang, Tongwei, Jia, Peina, Yan, Ruiying, Li, Shuli, Zou, Ruitao, Xue, Yuhua, Dai, Liming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887902/
https://www.ncbi.nlm.nih.gov/pubmed/27246808
http://dx.doi.org/10.1038/srep26961
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author Qi, Lei
Lv, Xiujuan
Zhang, Tongwei
Jia, Peina
Yan, Ruiying
Li, Shuli
Zou, Ruitao
Xue, Yuhua
Dai, Liming
author_facet Qi, Lei
Lv, Xiujuan
Zhang, Tongwei
Jia, Peina
Yan, Ruiying
Li, Shuli
Zou, Ruitao
Xue, Yuhua
Dai, Liming
author_sort Qi, Lei
collection PubMed
description A variety of nanomaterials have been developed for ocular diseases. The ability of these nanomaterials to pass through the blood-ocular barrier and their biocompatibility are essential characteristics that must be considered. Bacterial magnetosomes (BMs) are a type of biogenic magnetic nanomaterials synthesized by magnetotactic bacteria. Due to their unique biomolecular membrane shell and narrow size distribution of approximately 30 nm, BMs can pass through the blood-brain barrier. The similarity of the blood-ocular barrier to the blood-brain barrier suggests that BMs have great potential as treatments for ocular diseases. In this work, BMs were isolated from magnetotactic bacteria and evaluated in various cytotoxicity and genotoxicity studies in human retinal pigment epithelium (ARPE-19) cells. The BMs entered ARPE-19 cells by endocytosis after a 6-h incubation and displayed much lower cytotoxicity than chemically synthesized magnetic nanoparticles (MNPs). MNPs exhibited significantly higher genotoxicity than BMs and promoted the expression of Bax (the programmed cell death acceleration protein) and the induction of greater cell necrosis. In BM-treated cells, apoptosis tended to be suppressed via increased expression of the Bcl-2 protein. In conclusion, BMs display excellent biocompatibility and potential for use in the treatment of ocular diseases.
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spelling pubmed-48879022016-06-09 Cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells Qi, Lei Lv, Xiujuan Zhang, Tongwei Jia, Peina Yan, Ruiying Li, Shuli Zou, Ruitao Xue, Yuhua Dai, Liming Sci Rep Article A variety of nanomaterials have been developed for ocular diseases. The ability of these nanomaterials to pass through the blood-ocular barrier and their biocompatibility are essential characteristics that must be considered. Bacterial magnetosomes (BMs) are a type of biogenic magnetic nanomaterials synthesized by magnetotactic bacteria. Due to their unique biomolecular membrane shell and narrow size distribution of approximately 30 nm, BMs can pass through the blood-brain barrier. The similarity of the blood-ocular barrier to the blood-brain barrier suggests that BMs have great potential as treatments for ocular diseases. In this work, BMs were isolated from magnetotactic bacteria and evaluated in various cytotoxicity and genotoxicity studies in human retinal pigment epithelium (ARPE-19) cells. The BMs entered ARPE-19 cells by endocytosis after a 6-h incubation and displayed much lower cytotoxicity than chemically synthesized magnetic nanoparticles (MNPs). MNPs exhibited significantly higher genotoxicity than BMs and promoted the expression of Bax (the programmed cell death acceleration protein) and the induction of greater cell necrosis. In BM-treated cells, apoptosis tended to be suppressed via increased expression of the Bcl-2 protein. In conclusion, BMs display excellent biocompatibility and potential for use in the treatment of ocular diseases. Nature Publishing Group 2016-06-01 /pmc/articles/PMC4887902/ /pubmed/27246808 http://dx.doi.org/10.1038/srep26961 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Qi, Lei
Lv, Xiujuan
Zhang, Tongwei
Jia, Peina
Yan, Ruiying
Li, Shuli
Zou, Ruitao
Xue, Yuhua
Dai, Liming
Cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells
title Cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells
title_full Cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells
title_fullStr Cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells
title_full_unstemmed Cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells
title_short Cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells
title_sort cytotoxicity and genotoxicity of bacterial magnetosomes against human retinal pigment epithelium cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887902/
https://www.ncbi.nlm.nih.gov/pubmed/27246808
http://dx.doi.org/10.1038/srep26961
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