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Gene‐specific amplicons from metagenomes as an alternative to directed evolution for enzyme screening: a case study using phenylacetaldehyde reductases

Screening gene‐specific amplicons from metagenomes (S‐GAM) is a highly promising technique for the isolation of genes encoding enzymes for biochemical and industrial applications. From metagenomes, we isolated phenylacetaldehyde reductase (par) genes, which code for an enzyme that catalyzes the prod...

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Detalles Bibliográficos
Autores principales: Itoh, Nobuya, Kazama, Miki, Takeuchi, Nami, Isotani, Kentaro, Kurokawa, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887972/
https://www.ncbi.nlm.nih.gov/pubmed/27419059
http://dx.doi.org/10.1002/2211-5463.12067