Light-induced nuclear export reveals rapid dynamics of epigenetic modifications

We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylat...

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Detalles Bibliográficos
Autores principales: Yumerefendi, Hayretin, Lerner, Andrew Michael, Zimmerman, Seth Parker, Hahn, Klaus, Bear, James E, Strahl, Brian D., Kuhlman, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888063/
https://www.ncbi.nlm.nih.gov/pubmed/27089030
http://dx.doi.org/10.1038/nchembio.2068
Descripción
Sumario:We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.

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