Light-induced nuclear export reveals rapid dynamics of epigenetic modifications

We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylat...

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Detalles Bibliográficos
Autores principales: Yumerefendi, Hayretin, Lerner, Andrew Michael, Zimmerman, Seth Parker, Hahn, Klaus, Bear, James E, Strahl, Brian D., Kuhlman, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888063/
https://www.ncbi.nlm.nih.gov/pubmed/27089030
http://dx.doi.org/10.1038/nchembio.2068
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author Yumerefendi, Hayretin
Lerner, Andrew Michael
Zimmerman, Seth Parker
Hahn, Klaus
Bear, James E
Strahl, Brian D.
Kuhlman, Brian
author_facet Yumerefendi, Hayretin
Lerner, Andrew Michael
Zimmerman, Seth Parker
Hahn, Klaus
Bear, James E
Strahl, Brian D.
Kuhlman, Brian
author_sort Yumerefendi, Hayretin
collection PubMed
description We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.
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spelling pubmed-48880632016-10-18 Light-induced nuclear export reveals rapid dynamics of epigenetic modifications Yumerefendi, Hayretin Lerner, Andrew Michael Zimmerman, Seth Parker Hahn, Klaus Bear, James E Strahl, Brian D. Kuhlman, Brian Nat Chem Biol Article We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation. 2016-04-18 2016-06 /pmc/articles/PMC4888063/ /pubmed/27089030 http://dx.doi.org/10.1038/nchembio.2068 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Yumerefendi, Hayretin
Lerner, Andrew Michael
Zimmerman, Seth Parker
Hahn, Klaus
Bear, James E
Strahl, Brian D.
Kuhlman, Brian
Light-induced nuclear export reveals rapid dynamics of epigenetic modifications
title Light-induced nuclear export reveals rapid dynamics of epigenetic modifications
title_full Light-induced nuclear export reveals rapid dynamics of epigenetic modifications
title_fullStr Light-induced nuclear export reveals rapid dynamics of epigenetic modifications
title_full_unstemmed Light-induced nuclear export reveals rapid dynamics of epigenetic modifications
title_short Light-induced nuclear export reveals rapid dynamics of epigenetic modifications
title_sort light-induced nuclear export reveals rapid dynamics of epigenetic modifications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888063/
https://www.ncbi.nlm.nih.gov/pubmed/27089030
http://dx.doi.org/10.1038/nchembio.2068
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