Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds

Micro-plasma is a possible alternative treatment for wound management. The effect of micro-plasma on wound healing depends on its composition and temperature. The authors previously developed a capillary-tube-based micro-plasma system that can generate micro-plasma with a high nitric oxide-containin...

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Autores principales: Shao, Pei-Lin, Liao, Jiunn-Der, Wong, Tak-Wah, Wang, Yi-Cheng, Leu, Steve, Yip, Hon-Kan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889145/
https://www.ncbi.nlm.nih.gov/pubmed/27248979
http://dx.doi.org/10.1371/journal.pone.0156699
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author Shao, Pei-Lin
Liao, Jiunn-Der
Wong, Tak-Wah
Wang, Yi-Cheng
Leu, Steve
Yip, Hon-Kan
author_facet Shao, Pei-Lin
Liao, Jiunn-Der
Wong, Tak-Wah
Wang, Yi-Cheng
Leu, Steve
Yip, Hon-Kan
author_sort Shao, Pei-Lin
collection PubMed
description Micro-plasma is a possible alternative treatment for wound management. The effect of micro-plasma on wound healing depends on its composition and temperature. The authors previously developed a capillary-tube-based micro-plasma system that can generate micro-plasma with a high nitric oxide-containing species composition and mild working temperature. Here, the efficacy of micro-plasma treatment on wound healing in a laser-induced skin wound mouse model was investigated. A partial thickness wound was created in the back skin of each mouse and then treated with micro-plasma. Non-invasive methods, namely wound closure kinetics, optical coherence tomography (OCT), and laser Doppler scanning, were used to measure the healing efficiency in the wound area. Neo-tissue growth and the expressions of matrix metallopeptidase-3 (MMP-3) and laminin in the wound area were assessed using histological and immunohistochemistry (IHC) analysis. The results show that micro-plasma treatment promoted wound healing. Micro-plasma treatment significantly reduced the wound bed region. The OCT images and histological analysis indicates more pronounced tissue regrowth in the wound bed region after micro-plasma treatment. The laser Doppler images shows that micro-plasma treatment promoted blood flow in the wound bed region. The IHC results show that the level of laminin increased in the wound bed region after micro-plasma treatment, whereas the level of MMP-3 decreased. Based on these results, micro-plasma has potential to be used to promote the healing of skin wounds clinically.
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spelling pubmed-48891452016-06-10 Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds Shao, Pei-Lin Liao, Jiunn-Der Wong, Tak-Wah Wang, Yi-Cheng Leu, Steve Yip, Hon-Kan PLoS One Research Article Micro-plasma is a possible alternative treatment for wound management. The effect of micro-plasma on wound healing depends on its composition and temperature. The authors previously developed a capillary-tube-based micro-plasma system that can generate micro-plasma with a high nitric oxide-containing species composition and mild working temperature. Here, the efficacy of micro-plasma treatment on wound healing in a laser-induced skin wound mouse model was investigated. A partial thickness wound was created in the back skin of each mouse and then treated with micro-plasma. Non-invasive methods, namely wound closure kinetics, optical coherence tomography (OCT), and laser Doppler scanning, were used to measure the healing efficiency in the wound area. Neo-tissue growth and the expressions of matrix metallopeptidase-3 (MMP-3) and laminin in the wound area were assessed using histological and immunohistochemistry (IHC) analysis. The results show that micro-plasma treatment promoted wound healing. Micro-plasma treatment significantly reduced the wound bed region. The OCT images and histological analysis indicates more pronounced tissue regrowth in the wound bed region after micro-plasma treatment. The laser Doppler images shows that micro-plasma treatment promoted blood flow in the wound bed region. The IHC results show that the level of laminin increased in the wound bed region after micro-plasma treatment, whereas the level of MMP-3 decreased. Based on these results, micro-plasma has potential to be used to promote the healing of skin wounds clinically. Public Library of Science 2016-06-01 /pmc/articles/PMC4889145/ /pubmed/27248979 http://dx.doi.org/10.1371/journal.pone.0156699 Text en © 2016 Shao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shao, Pei-Lin
Liao, Jiunn-Der
Wong, Tak-Wah
Wang, Yi-Cheng
Leu, Steve
Yip, Hon-Kan
Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds
title Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds
title_full Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds
title_fullStr Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds
title_full_unstemmed Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds
title_short Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds
title_sort enhancement of wound healing by non-thermal n(2)/ar micro-plasma exposure in mice with fractional-co(2)-laser-induced wounds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889145/
https://www.ncbi.nlm.nih.gov/pubmed/27248979
http://dx.doi.org/10.1371/journal.pone.0156699
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