Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds
Micro-plasma is a possible alternative treatment for wound management. The effect of micro-plasma on wound healing depends on its composition and temperature. The authors previously developed a capillary-tube-based micro-plasma system that can generate micro-plasma with a high nitric oxide-containin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889145/ https://www.ncbi.nlm.nih.gov/pubmed/27248979 http://dx.doi.org/10.1371/journal.pone.0156699 |
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author | Shao, Pei-Lin Liao, Jiunn-Der Wong, Tak-Wah Wang, Yi-Cheng Leu, Steve Yip, Hon-Kan |
author_facet | Shao, Pei-Lin Liao, Jiunn-Der Wong, Tak-Wah Wang, Yi-Cheng Leu, Steve Yip, Hon-Kan |
author_sort | Shao, Pei-Lin |
collection | PubMed |
description | Micro-plasma is a possible alternative treatment for wound management. The effect of micro-plasma on wound healing depends on its composition and temperature. The authors previously developed a capillary-tube-based micro-plasma system that can generate micro-plasma with a high nitric oxide-containing species composition and mild working temperature. Here, the efficacy of micro-plasma treatment on wound healing in a laser-induced skin wound mouse model was investigated. A partial thickness wound was created in the back skin of each mouse and then treated with micro-plasma. Non-invasive methods, namely wound closure kinetics, optical coherence tomography (OCT), and laser Doppler scanning, were used to measure the healing efficiency in the wound area. Neo-tissue growth and the expressions of matrix metallopeptidase-3 (MMP-3) and laminin in the wound area were assessed using histological and immunohistochemistry (IHC) analysis. The results show that micro-plasma treatment promoted wound healing. Micro-plasma treatment significantly reduced the wound bed region. The OCT images and histological analysis indicates more pronounced tissue regrowth in the wound bed region after micro-plasma treatment. The laser Doppler images shows that micro-plasma treatment promoted blood flow in the wound bed region. The IHC results show that the level of laminin increased in the wound bed region after micro-plasma treatment, whereas the level of MMP-3 decreased. Based on these results, micro-plasma has potential to be used to promote the healing of skin wounds clinically. |
format | Online Article Text |
id | pubmed-4889145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48891452016-06-10 Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds Shao, Pei-Lin Liao, Jiunn-Der Wong, Tak-Wah Wang, Yi-Cheng Leu, Steve Yip, Hon-Kan PLoS One Research Article Micro-plasma is a possible alternative treatment for wound management. The effect of micro-plasma on wound healing depends on its composition and temperature. The authors previously developed a capillary-tube-based micro-plasma system that can generate micro-plasma with a high nitric oxide-containing species composition and mild working temperature. Here, the efficacy of micro-plasma treatment on wound healing in a laser-induced skin wound mouse model was investigated. A partial thickness wound was created in the back skin of each mouse and then treated with micro-plasma. Non-invasive methods, namely wound closure kinetics, optical coherence tomography (OCT), and laser Doppler scanning, were used to measure the healing efficiency in the wound area. Neo-tissue growth and the expressions of matrix metallopeptidase-3 (MMP-3) and laminin in the wound area were assessed using histological and immunohistochemistry (IHC) analysis. The results show that micro-plasma treatment promoted wound healing. Micro-plasma treatment significantly reduced the wound bed region. The OCT images and histological analysis indicates more pronounced tissue regrowth in the wound bed region after micro-plasma treatment. The laser Doppler images shows that micro-plasma treatment promoted blood flow in the wound bed region. The IHC results show that the level of laminin increased in the wound bed region after micro-plasma treatment, whereas the level of MMP-3 decreased. Based on these results, micro-plasma has potential to be used to promote the healing of skin wounds clinically. Public Library of Science 2016-06-01 /pmc/articles/PMC4889145/ /pubmed/27248979 http://dx.doi.org/10.1371/journal.pone.0156699 Text en © 2016 Shao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shao, Pei-Lin Liao, Jiunn-Der Wong, Tak-Wah Wang, Yi-Cheng Leu, Steve Yip, Hon-Kan Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds |
title | Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds |
title_full | Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds |
title_fullStr | Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds |
title_full_unstemmed | Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds |
title_short | Enhancement of Wound Healing by Non-Thermal N(2)/Ar Micro-Plasma Exposure in Mice with Fractional-CO(2)-Laser-Induced Wounds |
title_sort | enhancement of wound healing by non-thermal n(2)/ar micro-plasma exposure in mice with fractional-co(2)-laser-induced wounds |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889145/ https://www.ncbi.nlm.nih.gov/pubmed/27248979 http://dx.doi.org/10.1371/journal.pone.0156699 |
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