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Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats

Intrathecal injection of bombesin (BBS) promoted hypertensive and sympathoexcitatory effects in normotensive (NT) rats. However, the involvement of rostral ventrolateral medulla (RVLM) in these responses is still unclear. In the present study, we investigated: (1) the effects of BBS injected bilater...

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Autores principales: Pinto, Izabella S., Mourão, Aline A., da Silva, Elaine F., Camargo, Amanda S., Marques, Stefanne M., Gomes, Karina P., Fajemiroye, James O., da Silva Reis, Angela A., Rebelo, Ana C. S., Ferreira-Neto, Marcos L., Rosa, Daniel A., Freiria-Oliveira, André H., Castro, Carlos H., Colombari, Eduardo, Colugnati, Diego B., Pedrino, Gustavo R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889888/
https://www.ncbi.nlm.nih.gov/pubmed/27313544
http://dx.doi.org/10.3389/fphys.2016.00205
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author Pinto, Izabella S.
Mourão, Aline A.
da Silva, Elaine F.
Camargo, Amanda S.
Marques, Stefanne M.
Gomes, Karina P.
Fajemiroye, James O.
da Silva Reis, Angela A.
Rebelo, Ana C. S.
Ferreira-Neto, Marcos L.
Rosa, Daniel A.
Freiria-Oliveira, André H.
Castro, Carlos H.
Colombari, Eduardo
Colugnati, Diego B.
Pedrino, Gustavo R.
author_facet Pinto, Izabella S.
Mourão, Aline A.
da Silva, Elaine F.
Camargo, Amanda S.
Marques, Stefanne M.
Gomes, Karina P.
Fajemiroye, James O.
da Silva Reis, Angela A.
Rebelo, Ana C. S.
Ferreira-Neto, Marcos L.
Rosa, Daniel A.
Freiria-Oliveira, André H.
Castro, Carlos H.
Colombari, Eduardo
Colugnati, Diego B.
Pedrino, Gustavo R.
author_sort Pinto, Izabella S.
collection PubMed
description Intrathecal injection of bombesin (BBS) promoted hypertensive and sympathoexcitatory effects in normotensive (NT) rats. However, the involvement of rostral ventrolateral medulla (RVLM) in these responses is still unclear. In the present study, we investigated: (1) the effects of BBS injected bilaterally into RVLM on cardiorespiratory and sympathetic activity in NT and spontaneously hypertensive rats (SHR); (2) the contribution of RVLM BBS type 1 receptors (BB(1)) to the maintenance of hypertension in SHR. Urethane-anesthetized rats (1.2 g · kg(−1), i.v.) were instrumented to record mean arterial pressure (MAP), diaphragm (DIA) motor, and renal sympathetic nerve activity (RSNA). In NT rats and SHR, BBS (0.3 mM) nanoinjected into RVLM increased MAP (33.9 ± 6.6 and 37.1 ± 4.5 mmHg, respectively; p < 0.05) and RSNA (97.8 ± 12.9 and 84.5 ± 18.1%, respectively; p < 0.05). In SHR, BBS also increased DIA burst amplitude (115.3 ± 22.7%; p < 0.05). BB(1) receptors antagonist (BIM-23127; 3 mM) reduced MAP (–19.9 ± 4.4 mmHg; p < 0.05) and RSNA (−17.7 ± 3.8%; p < 0.05) in SHR, but not in NT rats (−2.5 ± 2.8 mmHg; −2.7 ± 5.6%, respectively). These results show that BBS can evoke sympathoexcitatory and pressor responses by activating RVLM BB(1) receptors. This pathway might be involved in the maintenance of high levels of arterial blood pressure in SHR.
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spelling pubmed-48898882016-06-16 Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats Pinto, Izabella S. Mourão, Aline A. da Silva, Elaine F. Camargo, Amanda S. Marques, Stefanne M. Gomes, Karina P. Fajemiroye, James O. da Silva Reis, Angela A. Rebelo, Ana C. S. Ferreira-Neto, Marcos L. Rosa, Daniel A. Freiria-Oliveira, André H. Castro, Carlos H. Colombari, Eduardo Colugnati, Diego B. Pedrino, Gustavo R. Front Physiol Physiology Intrathecal injection of bombesin (BBS) promoted hypertensive and sympathoexcitatory effects in normotensive (NT) rats. However, the involvement of rostral ventrolateral medulla (RVLM) in these responses is still unclear. In the present study, we investigated: (1) the effects of BBS injected bilaterally into RVLM on cardiorespiratory and sympathetic activity in NT and spontaneously hypertensive rats (SHR); (2) the contribution of RVLM BBS type 1 receptors (BB(1)) to the maintenance of hypertension in SHR. Urethane-anesthetized rats (1.2 g · kg(−1), i.v.) were instrumented to record mean arterial pressure (MAP), diaphragm (DIA) motor, and renal sympathetic nerve activity (RSNA). In NT rats and SHR, BBS (0.3 mM) nanoinjected into RVLM increased MAP (33.9 ± 6.6 and 37.1 ± 4.5 mmHg, respectively; p < 0.05) and RSNA (97.8 ± 12.9 and 84.5 ± 18.1%, respectively; p < 0.05). In SHR, BBS also increased DIA burst amplitude (115.3 ± 22.7%; p < 0.05). BB(1) receptors antagonist (BIM-23127; 3 mM) reduced MAP (–19.9 ± 4.4 mmHg; p < 0.05) and RSNA (−17.7 ± 3.8%; p < 0.05) in SHR, but not in NT rats (−2.5 ± 2.8 mmHg; −2.7 ± 5.6%, respectively). These results show that BBS can evoke sympathoexcitatory and pressor responses by activating RVLM BB(1) receptors. This pathway might be involved in the maintenance of high levels of arterial blood pressure in SHR. Frontiers Media S.A. 2016-06-02 /pmc/articles/PMC4889888/ /pubmed/27313544 http://dx.doi.org/10.3389/fphys.2016.00205 Text en Copyright © 2016 Pinto, Mourão, da Silva, Camargo, Marques, Gomes, Fajemiroye, da Silva Reis, Rebelo, Ferreira-Neto, Rosa, Freiria-Oliveira, Castro, Colombari, Colugnati and Pedrino. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Pinto, Izabella S.
Mourão, Aline A.
da Silva, Elaine F.
Camargo, Amanda S.
Marques, Stefanne M.
Gomes, Karina P.
Fajemiroye, James O.
da Silva Reis, Angela A.
Rebelo, Ana C. S.
Ferreira-Neto, Marcos L.
Rosa, Daniel A.
Freiria-Oliveira, André H.
Castro, Carlos H.
Colombari, Eduardo
Colugnati, Diego B.
Pedrino, Gustavo R.
Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats
title Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats
title_full Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats
title_fullStr Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats
title_full_unstemmed Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats
title_short Blockade of Rostral Ventrolateral Medulla (RVLM) Bombesin Receptor Type 1 Decreases Blood Pressure and Sympathetic Activity in Anesthetized Spontaneously Hypertensive Rats
title_sort blockade of rostral ventrolateral medulla (rvlm) bombesin receptor type 1 decreases blood pressure and sympathetic activity in anesthetized spontaneously hypertensive rats
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889888/
https://www.ncbi.nlm.nih.gov/pubmed/27313544
http://dx.doi.org/10.3389/fphys.2016.00205
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