Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay

Hammerhead ribozymes are self-cleaving RNA molecules capable of regulating gene expression in living cells. Their cleavage performance is strongly influenced by intra-molecular loop–loop interactions, a feature not readily accessible through modern prediction algorithms. Ribozyme engineering and eff...

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Detalles Bibliográficos
Autores principales: Ausländer, Simon, Fuchs, David, Hürlemann, Samuel, Ausländer, David, Fussenegger, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889925/
https://www.ncbi.nlm.nih.gov/pubmed/26939886
http://dx.doi.org/10.1093/nar/gkw117

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889925/
https://www.ncbi.nlm.nih.gov/pubmed/26939886
http://dx.doi.org/10.1093/nar/gkw117

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