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Long-range transcriptional interference in E. coli used to construct a dual positive selection system for genetic switches

We have investigated transcriptional interference between convergent genes in E. coli and demonstrate substantial interference for inter-promoter distances of as far as 3 kb. Interference can be elicited by both strong σ(70) dependent and T7 promoters. In the presented design, a strong promoter driv...

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Detalles Bibliográficos
Autores principales: Hoffmann, Stefan A., Kruse, Sabrina M., Arndt, Katja M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889929/
https://www.ncbi.nlm.nih.gov/pubmed/26932362
http://dx.doi.org/10.1093/nar/gkw125