Cargando…
Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine
The long noncoding RNA Gomafu/MIAT/Rncr2 is thought to function in retinal cell specification, stem cell differentiation and the control of alternative splicing. To further investigate physiological functions of Gomafu, we created mouse knockout (KO) model that completely lacks the Gomafu gene. The...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890022/ https://www.ncbi.nlm.nih.gov/pubmed/27251103 http://dx.doi.org/10.1038/srep27204 |
_version_ | 1782435053500891136 |
---|---|
author | Ip, Joanna Y. Sone, Masamitsu Nashiki, Chieko Pan, Qun Kitaichi, Kiyoyuki Yanaka, Kaori Abe, Takaya Takao, Keizo Miyakawa, Tsuyoshi Blencowe, Benjamin J. Nakagawa, Shinichi |
author_facet | Ip, Joanna Y. Sone, Masamitsu Nashiki, Chieko Pan, Qun Kitaichi, Kiyoyuki Yanaka, Kaori Abe, Takaya Takao, Keizo Miyakawa, Tsuyoshi Blencowe, Benjamin J. Nakagawa, Shinichi |
author_sort | Ip, Joanna Y. |
collection | PubMed |
description | The long noncoding RNA Gomafu/MIAT/Rncr2 is thought to function in retinal cell specification, stem cell differentiation and the control of alternative splicing. To further investigate physiological functions of Gomafu, we created mouse knockout (KO) model that completely lacks the Gomafu gene. The KO mice did not exhibit any developmental deficits. However, behavioral tests revealed that the KO mice are hyperactive. This hyperactive behavior was enhanced when the KO mice were treated with the psychostimulant methamphetamine, which was associated with an increase in dopamine release in the nucleus accumbens. RNA sequencing analyses identified a small number of genes affected by the deficiency of Gomafu, a subset of which are known to have important neurobiological functions. These observations suggest that Gomafu modifies mouse behavior thorough a mild modulation of gene expression and/or alternative splicing of target genes. |
format | Online Article Text |
id | pubmed-4890022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48900222016-06-09 Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine Ip, Joanna Y. Sone, Masamitsu Nashiki, Chieko Pan, Qun Kitaichi, Kiyoyuki Yanaka, Kaori Abe, Takaya Takao, Keizo Miyakawa, Tsuyoshi Blencowe, Benjamin J. Nakagawa, Shinichi Sci Rep Article The long noncoding RNA Gomafu/MIAT/Rncr2 is thought to function in retinal cell specification, stem cell differentiation and the control of alternative splicing. To further investigate physiological functions of Gomafu, we created mouse knockout (KO) model that completely lacks the Gomafu gene. The KO mice did not exhibit any developmental deficits. However, behavioral tests revealed that the KO mice are hyperactive. This hyperactive behavior was enhanced when the KO mice were treated with the psychostimulant methamphetamine, which was associated with an increase in dopamine release in the nucleus accumbens. RNA sequencing analyses identified a small number of genes affected by the deficiency of Gomafu, a subset of which are known to have important neurobiological functions. These observations suggest that Gomafu modifies mouse behavior thorough a mild modulation of gene expression and/or alternative splicing of target genes. Nature Publishing Group 2016-06-02 /pmc/articles/PMC4890022/ /pubmed/27251103 http://dx.doi.org/10.1038/srep27204 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ip, Joanna Y. Sone, Masamitsu Nashiki, Chieko Pan, Qun Kitaichi, Kiyoyuki Yanaka, Kaori Abe, Takaya Takao, Keizo Miyakawa, Tsuyoshi Blencowe, Benjamin J. Nakagawa, Shinichi Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine |
title | Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine |
title_full | Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine |
title_fullStr | Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine |
title_full_unstemmed | Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine |
title_short | Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine |
title_sort | gomafu lncrna knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890022/ https://www.ncbi.nlm.nih.gov/pubmed/27251103 http://dx.doi.org/10.1038/srep27204 |
work_keys_str_mv | AT ipjoannay gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT sonemasamitsu gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT nashikichieko gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT panqun gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT kitaichikiyoyuki gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT yanakakaori gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT abetakaya gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT takaokeizo gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT miyakawatsuyoshi gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT blencowebenjaminj gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine AT nakagawashinichi gomafulncrnaknockoutmiceexhibitmildhyperactivitywithenhancedresponsivenesstothepsychostimulantmethamphetamine |