Detection of RNA-DNA association by a proximity ligation-based method

We describe a proximity ligation assay (PLA)-based method of assessing association of DNA and RNA in single cells during the cell cycle. Pulse-labeling of DNA with EdU and RNA with BrU and testing their close proximity by PLA demonstrates that RNA synthesis in individual cells resumes about 30–45 mi...

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Detalles Bibliográficos
Autores principales: Petruk, Svetlana, Fenstermaker, Tyler K., Black, Kathryn L., Brock, Hugh W., Mazo, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891662/
https://www.ncbi.nlm.nih.gov/pubmed/27256324
http://dx.doi.org/10.1038/srep27313

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891662/
https://www.ncbi.nlm.nih.gov/pubmed/27256324
http://dx.doi.org/10.1038/srep27313

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