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Detection of RNA-DNA association by a proximity ligation-based method

We describe a proximity ligation assay (PLA)-based method of assessing association of DNA and RNA in single cells during the cell cycle. Pulse-labeling of DNA with EdU and RNA with BrU and testing their close proximity by PLA demonstrates that RNA synthesis in individual cells resumes about 30–45 mi...

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Detalles Bibliográficos
Autores principales:	Petruk, Svetlana, Fenstermaker, Tyler K., Black, Kathryn L., Brock, Hugh W., Mazo, Alexander
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891662/ https://www.ncbi.nlm.nih.gov/pubmed/27256324 http://dx.doi.org/10.1038/srep27313

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