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Conessine Interferes with Oxidative Stress-Induced C2C12 Myoblast Cell Death through Inhibition of Autophagic Flux

Conessine, a steroidal alkaloid isolated from Holarrhena floribunda, has anti-malarial activity and interacts with the histamine H3 receptor. However, the cellular effects of conessine are poorly understood. Accordingly, we evaluated the involvement of conessine in the regulation of autophagy. We se...

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Autores principales: Kim, Hyunju, Lee, Kang Il, Jang, Minsu, Namkoong, Sim, Park, Rackhyun, Ju, Hyunwoo, Choi, Inho, Oh, Won Keun, Park, Junsoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892631/
https://www.ncbi.nlm.nih.gov/pubmed/27257813
http://dx.doi.org/10.1371/journal.pone.0157096
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author Kim, Hyunju
Lee, Kang Il
Jang, Minsu
Namkoong, Sim
Park, Rackhyun
Ju, Hyunwoo
Choi, Inho
Oh, Won Keun
Park, Junsoo
author_facet Kim, Hyunju
Lee, Kang Il
Jang, Minsu
Namkoong, Sim
Park, Rackhyun
Ju, Hyunwoo
Choi, Inho
Oh, Won Keun
Park, Junsoo
author_sort Kim, Hyunju
collection PubMed
description Conessine, a steroidal alkaloid isolated from Holarrhena floribunda, has anti-malarial activity and interacts with the histamine H3 receptor. However, the cellular effects of conessine are poorly understood. Accordingly, we evaluated the involvement of conessine in the regulation of autophagy. We searched natural compounds that modulate autophagy, and conessine was identified as an inhibitor of autophagic flux. Conessine treatment induced the formation of autophagosomes, and p62, an autophagic adapter, accumulated in the autophagosomes. Reactive oxygen species such as hydrogen peroxide (H(2)O(2)) result in muscle cell death by inducing excessive autophagic flux. Treatment with conessine inhibited H(2)O(2)-induced autophagic flux in C2C12 myoblast cells and also interfered with cell death. Our results indicate that conessine has the potential effect to inhibit muscle cell death by interfering with autophagic flux.
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spelling pubmed-48926312016-06-16 Conessine Interferes with Oxidative Stress-Induced C2C12 Myoblast Cell Death through Inhibition of Autophagic Flux Kim, Hyunju Lee, Kang Il Jang, Minsu Namkoong, Sim Park, Rackhyun Ju, Hyunwoo Choi, Inho Oh, Won Keun Park, Junsoo PLoS One Research Article Conessine, a steroidal alkaloid isolated from Holarrhena floribunda, has anti-malarial activity and interacts with the histamine H3 receptor. However, the cellular effects of conessine are poorly understood. Accordingly, we evaluated the involvement of conessine in the regulation of autophagy. We searched natural compounds that modulate autophagy, and conessine was identified as an inhibitor of autophagic flux. Conessine treatment induced the formation of autophagosomes, and p62, an autophagic adapter, accumulated in the autophagosomes. Reactive oxygen species such as hydrogen peroxide (H(2)O(2)) result in muscle cell death by inducing excessive autophagic flux. Treatment with conessine inhibited H(2)O(2)-induced autophagic flux in C2C12 myoblast cells and also interfered with cell death. Our results indicate that conessine has the potential effect to inhibit muscle cell death by interfering with autophagic flux. Public Library of Science 2016-06-03 /pmc/articles/PMC4892631/ /pubmed/27257813 http://dx.doi.org/10.1371/journal.pone.0157096 Text en © 2016 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kim, Hyunju
Lee, Kang Il
Jang, Minsu
Namkoong, Sim
Park, Rackhyun
Ju, Hyunwoo
Choi, Inho
Oh, Won Keun
Park, Junsoo
Conessine Interferes with Oxidative Stress-Induced C2C12 Myoblast Cell Death through Inhibition of Autophagic Flux
title Conessine Interferes with Oxidative Stress-Induced C2C12 Myoblast Cell Death through Inhibition of Autophagic Flux
title_full Conessine Interferes with Oxidative Stress-Induced C2C12 Myoblast Cell Death through Inhibition of Autophagic Flux
title_fullStr Conessine Interferes with Oxidative Stress-Induced C2C12 Myoblast Cell Death through Inhibition of Autophagic Flux
title_full_unstemmed Conessine Interferes with Oxidative Stress-Induced C2C12 Myoblast Cell Death through Inhibition of Autophagic Flux
title_short Conessine Interferes with Oxidative Stress-Induced C2C12 Myoblast Cell Death through Inhibition of Autophagic Flux
title_sort conessine interferes with oxidative stress-induced c2c12 myoblast cell death through inhibition of autophagic flux
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892631/
https://www.ncbi.nlm.nih.gov/pubmed/27257813
http://dx.doi.org/10.1371/journal.pone.0157096
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