Cargando…

The source of SYBR green master mix determines outcome of nucleic acid amplification reactions

BACKGROUND: Quantitative (q) PCR by amplification of nucleic acid with a fluorescent dye is widely used. Selection of adequate PCR reagents and devices is relevant to achieve reliable and consistent data. Our main objective was to test the robustness of different commercial SYBR green PCR mixes with...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Jianxin, Kemps-Mols, Berit, Spruyt-Gerritse, Marijke, Anholts, Jacqueline, Claas, Frans, Eikmans, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893258/
https://www.ncbi.nlm.nih.gov/pubmed/27259280
http://dx.doi.org/10.1186/s13104-016-2093-4