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The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
BACKGROUND: Quantitative (q) PCR by amplification of nucleic acid with a fluorescent dye is widely used. Selection of adequate PCR reagents and devices is relevant to achieve reliable and consistent data. Our main objective was to test the robustness of different commercial SYBR green PCR mixes with...
Autores principales: | Yang, Jianxin, Kemps-Mols, Berit, Spruyt-Gerritse, Marijke, Anholts, Jacqueline, Claas, Frans, Eikmans, Michael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893258/ https://www.ncbi.nlm.nih.gov/pubmed/27259280 http://dx.doi.org/10.1186/s13104-016-2093-4 |
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