IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly

In vivo homologous recombination holds the potential for optimal molecular cloning, however, current strategies require specialised bacterial strains or laborious protocols. Here, we exploit a recA-independent recombination pathway, present in widespread laboratory E.coli strains, to develop IVA (In...

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Detalles Bibliográficos
Autores principales: García-Nafría, Javier, Watson, Jake F., Greger, Ingo H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893743/
https://www.ncbi.nlm.nih.gov/pubmed/27264908
http://dx.doi.org/10.1038/srep27459

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893743/
https://www.ncbi.nlm.nih.gov/pubmed/27264908
http://dx.doi.org/10.1038/srep27459

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