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Application of a modified drop method for high-resolution pachytene chromosome spreads in two Phalaenopsis species

BACKGROUND: Preparation of good chromosome spreads without cytoplasmic contamination is the crucial step in cytogenetic mapping. To date, cytogenetic research in the Orchidaceae family has been carried out solely on mitotic metaphase chromosomes. Well-spread meiotic pachytene chromosomes can provide...

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Detalles Bibliográficos
Autores principales: Kuo, Yi-Tzu, Hsu, Hui-Lan, Yeh, Chih-Hsin, Chang, Song-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893830/
https://www.ncbi.nlm.nih.gov/pubmed/27275186
http://dx.doi.org/10.1186/s13039-016-0254-8