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Design of a Split Intein with Exceptional Protein Splicing Activity
[Image: see text] Protein trans-splicing (PTS) by split inteins has found widespread use in chemical biology and biotechnology. Herein, we describe the use of a consensus design approach to engineer a split intein with enhanced stability and activity that make it more robust than any known PTS syste...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894280/ https://www.ncbi.nlm.nih.gov/pubmed/26854538 http://dx.doi.org/10.1021/jacs.5b13528 |