A method to rapidly create protein aggregates in living cells

The accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors involved in their clearance. Here, we report a che...

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Autores principales: Miyazaki, Yusuke, Mizumoto, Kota, Dey, Gautam, Kudo, Takamasa, Perrino, John, Chen, Ling-chun, Meyer, Tobias, Wandless, Thomas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894968/
https://www.ncbi.nlm.nih.gov/pubmed/27229621
http://dx.doi.org/10.1038/ncomms11689
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author Miyazaki, Yusuke
Mizumoto, Kota
Dey, Gautam
Kudo, Takamasa
Perrino, John
Chen, Ling-chun
Meyer, Tobias
Wandless, Thomas J.
author_facet Miyazaki, Yusuke
Mizumoto, Kota
Dey, Gautam
Kudo, Takamasa
Perrino, John
Chen, Ling-chun
Meyer, Tobias
Wandless, Thomas J.
author_sort Miyazaki, Yusuke
collection PubMed
description The accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors involved in their clearance. Here, we report a chemically controllable fluorescent protein that enables us to rapidly produce small aggregates inside living cells on the order of seconds, as well as monitor the movement and coalescence of individual aggregates into larger structures. This method can be applied to diverse experimental systems, including live animals, and may prove valuable for understanding cellular responses and diseases associated with protein aggregates.
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spelling pubmed-48949682016-06-21 A method to rapidly create protein aggregates in living cells Miyazaki, Yusuke Mizumoto, Kota Dey, Gautam Kudo, Takamasa Perrino, John Chen, Ling-chun Meyer, Tobias Wandless, Thomas J. Nat Commun Article The accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors involved in their clearance. Here, we report a chemically controllable fluorescent protein that enables us to rapidly produce small aggregates inside living cells on the order of seconds, as well as monitor the movement and coalescence of individual aggregates into larger structures. This method can be applied to diverse experimental systems, including live animals, and may prove valuable for understanding cellular responses and diseases associated with protein aggregates. Nature Publishing Group 2016-05-27 /pmc/articles/PMC4894968/ /pubmed/27229621 http://dx.doi.org/10.1038/ncomms11689 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Miyazaki, Yusuke
Mizumoto, Kota
Dey, Gautam
Kudo, Takamasa
Perrino, John
Chen, Ling-chun
Meyer, Tobias
Wandless, Thomas J.
A method to rapidly create protein aggregates in living cells
title A method to rapidly create protein aggregates in living cells
title_full A method to rapidly create protein aggregates in living cells
title_fullStr A method to rapidly create protein aggregates in living cells
title_full_unstemmed A method to rapidly create protein aggregates in living cells
title_short A method to rapidly create protein aggregates in living cells
title_sort method to rapidly create protein aggregates in living cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894968/
https://www.ncbi.nlm.nih.gov/pubmed/27229621
http://dx.doi.org/10.1038/ncomms11689
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